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xtremey spin column kit  (Thermo Fisher)


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    Thermo Fisher xtremey spin column kit
    Xtremey Spin Column Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xtremey+spin+column+kit/pm08703016-105-7-11
    Average 90 stars, based on 1 article reviews
    xtremey spin column kit - by Bioz Stars, 2026-09
    90/100 stars

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    Labeling:

    Article Title: Identification of a mutation in a GATA binding site of the platelet glycoprotein Ibbeta promoter resulting in the Bernard-Soulier syndrome.
    Article Snippet: The oligonucleotides were labeled for 30 min at 37 °C in a reaction containing 3.5 pmol of oligonucleotide, 30 mCi of [g-32P]dATP (DuPont NEN) and 10 units of T4 polynucleotide kinase (New England Biolabs, Beverly, MA) in 70 mM Tris-HCl, pH 7.6, 10 mM MgCl2, 5 mM dithiothreitol. .. The labeled probes were purified using the Xtremey spin column kit (Pierce). .. Before adding probe, the binding reactions containing 3–12 mg of nuclear extract and 0.5 mg of poly(dI-dC)z(dI-dC) (Pharmacia Biotech Inc.) in 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM EDTA, 1 mM dithiothreitol, 15% glycerol were preincubated for 10 min at room temperature as described by Uzan et al. (27).

    Purification:

    Article Title: Identification of a mutation in a GATA binding site of the platelet glycoprotein Ibbeta promoter resulting in the Bernard-Soulier syndrome.
    Article Snippet: The oligonucleotides were labeled for 30 min at 37 °C in a reaction containing 3.5 pmol of oligonucleotide, 30 mCi of [g-32P]dATP (DuPont NEN) and 10 units of T4 polynucleotide kinase (New England Biolabs, Beverly, MA) in 70 mM Tris-HCl, pH 7.6, 10 mM MgCl2, 5 mM dithiothreitol. .. The labeled probes were purified using the Xtremey spin column kit (Pierce). .. Before adding probe, the binding reactions containing 3–12 mg of nuclear extract and 0.5 mg of poly(dI-dC)z(dI-dC) (Pharmacia Biotech Inc.) in 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM EDTA, 1 mM dithiothreitol, 15% glycerol were preincubated for 10 min at room temperature as described by Uzan et al. (27).



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    xtremey spin column kit  (Thermo Fisher)
    90
    Thermo Fisher xtremey spin column kit
    Xtremey Spin Column Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xtremey+spin+column+kit/pm08703016-105-7-11
    Average 90 stars, based on 1 article reviews
    xtremey spin column kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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